It is intended to provide an overview of: • Flow Cytometry and its associated applications • The basic components of the flow cytometer » This information can be used to individually sort or separate subpopulations of cells. Flow Cytometry » Flow Cytometry is the technical process that allows for the individual measurements of cell fluorescence and light scattering. Getting started analyzing your flow data cytometry with the time gate. Scientists use flow cytometry to differentiate between different types of cells or microscopic organisms. This process is performed at rates of thousands of cells per second. Flow Cytometry is a widely used method for cell analysis which, for the novice, can appear daunting due to the complexity of the experimental approach and data analysis. The time parameter measures the duration of each sample run. It is a tool used in many applications such as medical diagnostics or forensic pathology. By analyzing the time gate in relation to a scatter parameter like SSC or FSC, you can identify and remove periods of time during your run where micro bubbles, micro clogs, or dry air were introduced. This simple introductory guide has been written with such individuals in mind.

But gating in flow cytometry experiments is one of the most important parts of the process. A good place to start gating your flow data is by using the Time gate. Gating can be a scary process Because of its importance, gating can also be a scary process, especially if you don’t know that much about the cells you are analyzing – What if

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